In the above article [Hiranita T, Wilkinson DS, Hong WC, Zou M-F, Kopajtic TA, Soto PL, Lupica CR, Newman AH, and Katz JL (2014) J Pharmacol Exp Ther 349:297–309; doi:10.1124/jpet.113.212738], there are a number of errors that are addressed below.
On page 299, under Materials and Methods, in the section “Monoamine Transporter Binding Assays,” the line “(50,000g for the NET assay)” should be omitted. The sentence should read “Tissue was dissected and homogenized in buffer using a Brinkman Polytron (at setting 6 for 20 seconds) and was subsequently centrifuged at 20,000g for 10 minutes at 4° C.” In the section “σ1 and σ2 Receptor Binding Assay, lines 9 and 12, 50,000g should be 20,000g.
In Table 1, under the column “Tissue,” 40 mg/tube should be 8 mg/tube. Under the column “Incubation Buffer,” for muscarinic M1, “0.5 ml of buffer (10 mM Tris-HCl, 5.0 mM MgCl2)” should be “0.5 ml of buffer (10 mM Tris-HCl, 5.0 mM MgCl2, pH 7.4),” with the pH value added. In addition, for σ1 and σ2, “10 mM Tris-HCl with 0.32 M sucrose, pH 7.4” should be “10 mM Tris-HCl, pH 8.”
The online versions of this article have been corrected.
The authors regret these errors and for any inconvenience they may have caused.
- Copyright © 2014 by The American Society for Pharmacology and Experimental Therapeutics