Abstract
After the i.v. injection of tritium-labeled l-tmyptOphan and l-tymosine into mats, the specific activity of both compounds in plasma declined multiphasically. The specific activity of norepinephrine, dopamine and serotonin in brainstem and tele-diencephalon increased rapidly, surpassed the specific activity of the precursor amino acids in about 50 minutes and then declined slowly approaching the amino acid values. Simple equations were derived to calculate synthesis rates from the changes in the specific activities of the amines and amino acids. The calculated mates are consistent with previously reported values. With this new procedure, it will be possible to either correlate animal behavior with the rate of amine formation in various brain structures or to study the effect of drugs on brain monoamine synthesis rates.
Footnotes
- Received March 20, 1970.
- Accepted November 9, 1970.
- © 1971 by The Williams & Wilkins Co.
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