Abstract
When phosphorus-labeled DFP (3-4 millicuries P32 per millimol) is injected intravenously into rabbits, radiophosphorus is retained in relatively large amounts by kidney, liver and lung, while other organs take up only small or insignificant amounts. The specific retention of P32 depends on the dose administered, and on the length of survival. No correlation seems to exist between the cholinestera. se activity of rabbit organs, and their ability to retain DFP-derived phosphorus. No significant retention of P32 in any tissue was found when labeled diisopropylphosphate was administered.
Of the total amount of DFP-derived P32 retained in liver, lung and kidney, a fraction which reaches 100 per cent after four hours of survival is bound to tissue proteins.
In plasma of rabbits injected with labeled DFP, most of the P32 originally present is eliminated before regeneration of cholinesterase activity has started. In erythrocytes the elimination of P32 parallels the reappearance of cholinesterase, indicating the destruction of cells whose cholinesterase was inactivated by DFP and their replacement with new cells containing active esterase.
Footnotes
- Received October 5, 1949.
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