Abstract
A sensitive radioimmunoassay for d-tubocurarine (d-TC) has been developed by conjugating d-TC to bovine serum albumin. Rabbits were immunized in the foot pad with this immunogen (50 µg) for a period of two months. Antibodies induced by this immunization procedure were capable of recognizing d-TC. The specificities of these antibodies will be discussed. The radioimmunoassay developed depends upon a competition between unlabeled d-TC and a standard of labeled 3H-d-TC for the specific antibodies present in the rabbit antisera. The assay is extremely sensitive measuring as little as 5 ng of d-TC per ml and requires only 10 µl of serum or urine sampled. Serum concentration of d-TC in cats receiving 25 and 300 µg/kg i.v.and in subjects receiving 300 µg/kg i.v. were determined. The advantage of this type of assay over previous methods lies in its sensitivity and in the fact that no extraction from biological materials is required.
Footnotes
- Received August 14, 1972.
- Accepted October 30, 1972.
- © 1973 by The Williams & Wilkins Company
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