Abstract
An existing gas chromatographic method for estimating choline and acctylcholine concentrations in tissue extracts has been modified to allow the simultaneous estimation by radio gas chromatography of the specific activities of both compounds. The chemical specificity of this method has been demonstrated with gas chromatography-mass spectrometry. This technique has been applied to the study of acetyicholine turnover in vivo. Specific activities of choline and acetylcholine have been determined in the rat submaxillary and sublingual glands as a function of time, after the i.v. injection of phosphoryl(Me-14C) choline (25 µc/kg). A precursor-product relationship has been demonstrated by this approach. Preliminary findings indicate that such an approach is highly suitable for application to studies of biochemical mechanisms regulating cholinergic nerve activity.
Footnotes
- Received September 23, 1971.
- Accepted December 9, 1971.
- © 1972, by The Williams & Wilkins Company
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