Abstract
Parathion was incubated with rabbit-liver microsomes and a reduced nicotinamide adenine dinucleotide phosphate-generating system in an atmosphere of 18O2 or in a medium containing H218O. The products of the reactions were isolated and the 18O enrichment was determined by gas chromatography-mass spectrometry. The data clearly show that oxygen from 18O2 is retained in paraoxon, the product of the desulfuration reaction, and in diethylphosphate, the hydrolysis product of paraoxon, but not in diethylphosphorothionate, the product of the dearylation reaction. When the reaction is carried out in H218O, the label is found in diethylphosphorothionate but not paraoxon. With the results of these experiments, we have postulated a chemical mechanism for the mixed function oxidase-catalyzed metabolism of parathion to paraoxon and diethylphosphorothionate.
Footnotes
- Received April 22, 1971.
- Accepted July 26, 1971.
- © 1971 by The Williams & Wilkins Co.
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