Abstract

After the fluorescent localization of norepinephrine (NE), sections of the guinea-pig hypogastric ganglion have been counterstained for acetylcholinesterase (AChE). Neurons which contained high levels of AChE contained little, if any, noradrenergic fluorescence, and neurons which contained high levels of NE were invariably devoid of heavy AChE staining but sometimes stained lightly for AChE. Some neurons did not react strongly for either NE or AChE. The results support existing evidence for the presence of separate cholinergic and adrenergic nerves supplying the musculature of the vas deferens of the guinea pig.