Abstract
The preparation of d-tubocurarine-H3 has permitted studies of its localization at the motor endplate through techniques of autoradiography. Five microcuries of d-tubocurarine-H3, equivalent to a dose of 0.6 µg/g b.wt., were injected into the tail vein of Swiss mice. The animals were sacrificed at the end of 1 min by decapitation and the diaphragm was removed. The latter was fixed in formalin vapor and sections were mounted after freezing in albumin. Cut sections (30 µ) were stained by a modification of the Koelle method adapted to prevent loss of the water-soluble d-tubocurarine. Autoradiography was performed with NTB3 emulsion. Development followed 10 to 21 days of exposure at 4°C. Localization of d-tubocurarine-H3 at the endplate was confirmed by light microscopy. Occasional evidence of strands of radioactivity leading into synaptic areas which contain dense radioactivity is suggestive of added preterminal localization of the drug.
Footnotes
- Received August 1, 1966.
- Accepted February 7, 1967.
- © 1967 by The Williams & Wilkins Company
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