Abstract
The identification of phenothiazines and antihistaminics in body fluids through the use of thin-layer chromatography is described. The drugs are extracted at pH 9.0 from blood or urine into ethylene dichloride containing 10% isoamyl alcohol. The extracting solvent is evaporated in vacuo and the residue is dissolved in a small volume of ethanol, and is then spotted on thin-layer chromatoplates. Rf values and color reactions for 18 phenothiazines and related compounds and 8 sulfoxides and for 18 antihistaminics and chlordiazepoxide are given.
In order to demonstrate the applicability of the thin-layer technique to studies of the metabolism of the phenothiazines and antihistaminics as well as to their detection, the usual clinical doses of both types of drugs were given to patients and the original compound and/or their metabolites were detected and identified in the urine.
Footnotes
- Received September 14, 1962.
- Accepted November 26, 1962.
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