Abstract
Ethylenimino-C14-labeled TEM was synthesized from ethanolamine-C14. The in vivo metabolism of TEM-C14 was studied after intraperitoneal or intravenous administration to mice and the nature of its urinary metabolites investigated. The radioactivity found in blood samples of mice and rats administered ethylenimino-labeled TEM-C14 intravenously indicated that over 90 per cent of the radioactivity was removed from the blood within a few minutes after injection. Comparatively little radioactivity was found in the feces and exhaled carbon dioxide. Between 68 and 73 per cent of the injected radioactivity was excreted in the urine within 24 hours and only 4 to 6 per cent in the next 24 hours. Chromatographic separation on an ion exchange column revealed at least 16 urinary metabolites. Five major radioactive peaks in the eluant accounted for 74 per cent of the total radioactivity, the largest being 34 per cent. One of these major peaks was identified as creatinine. Less than 1 per cent of the urinary radioactivity was present as urea. None of the other major radioactive metabolites were normally occurring constituents of urine. The possibility of the unidentified urinary metabolites being alkylated products of amino acids or other normally occurring compounds has been discussed.
Footnotes
- Received January 13, 1958.
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