Abstract
The inhibitory effects of central and peripherally acting opioid agonists on intestinal permeability (PER) were evaluated during acute and chronic intestinal inflammation in mice. Inflammation was induced by the intragastric (p.o.) administration of one (acute) or two (chronic) doses of croton oil (CO), whereas controls received saline (SS). Intestinal PER was assessed by the blood-to-lumen transfer of51Cr-ethylenediaminetetraacetate (51Cr-EDTA). CO significantly increased PER during acute (2.5 times) and chronic (3.2 times) inflammation. The potency of s.c. morphine-inhibiting PER was enhanced 3.8 and 8.7 times in acute and chronic CO, whereas that of s.c. fentanyl was increased 2.0 and 4.3 times, respectively, compared with SS. Similarly, s.c. [d-Pen2,5]-enkephalin was 4.7 and 11.1 times more potent during acute and chronic CO, and theE max values of the dose-response curves increased 35% during inflammation. The potency of s.c. U50,488H was 5.6 (acute) and 6.7 times (chronic) greater compared with SS. All effects were reversed by specific antagonists. The i.p. administration of β-funaltrexamine differentially blocked morphine effects during acute and chronic CO, suggesting that the effects are mediated by different populations of functional μ-opioid receptors (OR). The increase in potencies of s.c. PL017 and ICI-204,448 during CO were comparable to those observed with fentanyl and U50,488H and their effects were antagonized by s.c. naloxone methiodide. Moreover, the potency of the agonists during inflammation was unaltered when administered i.c.v. The results show that intestinal inflammation enhances the effects of δ- > μ- > κ-opioid agonists on PER by activation of peripheral OR.
Footnotes
- Received June 1, 2000.
- Accepted October 10, 2000.
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Send reprint requests to: Margarita M. Puig, M.D., Ph.D., Department of Anaesthesiology, Hospital Universitario del Mar, Paseo Marı́timo 25, 08003 Barcelona, Spain. E-mail:86822{at}imas.imim.es
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This work was partially supported by grants from Comisión Interministerial de Ciencia y Tecnologı́a PM98-0155 and FIS 00/0658, Madrid; Fundació La Marató de TV3 2032/97 and Generalitat de Catalunya 1997SGR00342, Barcelona, Spain. Part of these results has been presented as a communication to the 2nd European Opioid Conference, Barcelona, Spain, April, 1999 and in the 8th European Society of Anaesthesiologists, Viena, Austria, April, 2000.
- The American Society for Pharmacology and Experimental Therapeutics
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