Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • For Subscribers
    • For Advertisers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • For Subscribers
    • For Advertisers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
OtherRENAL PHARMACOLOGY

Phosphate Excretion and Phosphate Transporter Messenger RNA in Uremic Rats Treated with Phosphonoformic Acid

David P. Brooks, Shujath M. Ali, Lisa C. Contino, Elwood Stack, Todd A. Fredrickson, John Feild and Richard M. Edwards
Journal of Pharmacology and Experimental Therapeutics June 1997, 281 (3) 1440-1445;
David P. Brooks
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shujath M. Ali
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lisa C. Contino
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elwood Stack
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Todd A. Fredrickson
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John Feild
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Richard M. Edwards
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The prevention of phosphate retention in chronic renal disease may reduce both renal osteodystrophy and disease progression. We evaluated the expression of the sodium-dependent phosphate transporter, NaPi-2, and the response to phosphonoformic acid (PFA) in rats with 5/6 nephrectomy-induced renal failure. Partial nephrectomy resulted in a significant proteinuria and reduced renal function. In addition, there was an ∼50% reduction in the expression of NaPi-2 mRNA. Treatment of rats for 48 hr with PFA (0.6% in glucose drinking fluid) had no effect on NaPi-2 mRNA; however, PFA resulted in a significant increase in fractional phosphate excretion in both normal (7 ± 0.5%vs. 3 ± 0.2%) and uremic (60 ± 4%vs. 36 ± 4%) rats. Plasma phosphate concentration was higher in uremic rats (2.5 ± 0.1 mM) compared with normal rats (1.9 ± 0.04 mM) but not in uremic rats treated with PFA (2.1 ± 0.04 mM). These data suggest that PFA can increase renal phosphate excretion independent of changes in phosphate transporter expression and prevent phosphate retention.

Footnotes

  • Send reprint requests to: David Brooks, Ph.D., SmithKline Beecham, Department of Renal Pharmacology, UW2521, P.O. Box 1539, King of Prussia, PA 19406-0939.

  • Abbreviations:
    PFA
    phosphonoformic acid
    NaPi
    sodium phosphate
    SGT
    sodium glucose cotransporter
    PCR
    polymerase chain reaction
    SDS
    sodium dodecyl sulfate
    GAPDH
    glyceraldehyde-3-phosphate dehydrogenase
    • Received August 26, 1996.
    • Accepted February 3, 1997.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

Log in using your username and password

Forgot your user name or password?

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 281, Issue 3
1 Jun 1997
  • Table of Contents
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Phosphate Excretion and Phosphate Transporter Messenger RNA in Uremic Rats Treated with Phosphonoformic Acid
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
Citation Tools
OtherRENAL PHARMACOLOGY

Phosphate Excretion and Phosphate Transporter Messenger RNA in Uremic Rats Treated with Phosphonoformic Acid

David P. Brooks, Shujath M. Ali, Lisa C. Contino, Elwood Stack, Todd A. Fredrickson, John Feild and Richard M. Edwards
Journal of Pharmacology and Experimental Therapeutics June 1, 1997, 281 (3) 1440-1445;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
OtherRENAL PHARMACOLOGY

Phosphate Excretion and Phosphate Transporter Messenger RNA in Uremic Rats Treated with Phosphonoformic Acid

David P. Brooks, Shujath M. Ali, Lisa C. Contino, Elwood Stack, Todd A. Fredrickson, John Feild and Richard M. Edwards
Journal of Pharmacology and Experimental Therapeutics June 1, 1997, 281 (3) 1440-1445;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Regulation of Renin Secretion Through Reversible Phosphorylation of Myosin by Myosin Light Chain Kinase and Protein Phosphatase Type 1
  • Interaction of l-Arginine Analogs withl-Arginine Uptake in Rat Renal Brush Border Membrane Vesicles
  • Effects of Trimetazidine on Lipid Peroxidation and Phosphorus Metabolites during Cold Storage and Reperfusion of Isolated Perfused Rat Kidneys
Show more RENAL PHARMACOLOGY

Similar Articles

  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2019 by the American Society for Pharmacology and Experimental Therapeutics