Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • For Subscribers
    • For Advertisers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • For Subscribers
    • For Advertisers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
OtherAUTONOMIC PHARMACOLOGY

Effects of LY215490, a Competitive α-Amino-3-Hydroxy-5-Methylisoxazole-4-Propionic Acid (AMPA) Receptor Antagonist, on the Micturition Reflex in the Rat

Mitsuharu Yoshiyama, James R. Roppolo and William C. de Groat
Journal of Pharmacology and Experimental Therapeutics February 1997, 280 (2) 894-904;
Mitsuharu Yoshiyama
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
James R. Roppolo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
William C. de Groat
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The effects of glutamate receptor antagonists on urinary bladder and external urethral sphincter- (EUS) electromyogram (EMG) activity were evaluated in unanesthetized decerebrate rats. In normal rats, LY215490, an α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor antagonist, in small i.v. doses (1–3 mg/kg) decreased bladder contraction amplitude (BC-Amp) by 29% and EUS-EMG by 41%; whereas a large dose (10 mg/kg) completely abolished bladder and EUS-EMG activity. LY215490 injected intrathecally in small doses (0.01–0.1 μg) decreased BC-Amp by 20% and EUS-EMG by 62%; whereas large doses (1–10 μg) completely abolished bladder and EUS-EMG activity. LY215490 (0.1 μg i.t.) increased bladder capacity by 28% and decreased voiding efficiency by 44%. Combined i.t. administration of small doses of LY215490 (0.1 μg) and MK-801 (1 μg), anN-methyl-d-aspartate (NMDA) receptor antagonist, which individually had little effect on BC-Amp, markedly suppressed bladder activity. In chronic spinal rats, LY215490 (10 mg/kg i.v.) abolished EUS-EMG activity and decreased BC-Amp by 41%. Intrathecal injections of LY215490 were also less effective in chronic spinal rats; a 10-μg dose producing only a partial block (53%) of BC-Amp, but complete block of EUS-EMG. In chronic spinal rats, MK-801 (1 mg/kg i.v.) abolished EUS-EMG activity and decreased BC-Amp by 36%. Pretreatment with MK-801 (1 mg/kg i.v.) did not enhance the effect of LY215490 on bladder activity in chronic spinal rats. These data suggest that AMPA glutamate receptors have a major role in the excitatory pathways controlling bladder and EUS activity in spinal cord intact rats. However, in chronic spinal rats, AMPA and NMDA receptors are essential for EUS reflexes, but are responsible for only a part of reflex bladder activity.

Footnotes

  • Send reprint requests to: Dr. Mitsuharu Yoshiyama, University of Pittsburgh, School of Medicine, Department of Pharmacology, E1303A Biomedical Science Tower, Pittsburgh, PA 15261.

  • ↵1 This work was supported by National Institutes of Health Grants DK-37241 and DK-49430 (W.D.).

  • Abbreviations:
    AMPA
    α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid
    NMDA
    N-methyl-d-aspartate
    CMG
    cystometrogram
    CSF
    cerebrospinal fluid
    EMG
    electromyogram
    EUS
    external urethral sphincter
    ICI
    intercontraction interval
    VT
    volume threshold
    VE
    voiding efficiency
    PMC
    pontine micturition center
    CNS
    central nervous system
    i.t.
    intrathecal
    • Received March 19, 1996.
    • Accepted October 7, 1996.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

Log in using your username and password

Forgot your user name or password?

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 280, Issue 2
1 Feb 1997
  • Table of Contents
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Effects of LY215490, a Competitive α-Amino-3-Hydroxy-5-Methylisoxazole-4-Propionic Acid (AMPA) Receptor Antagonist, on the Micturition Reflex in the Rat
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
Citation Tools
OtherAUTONOMIC PHARMACOLOGY

Effects of LY215490, a Competitive α-Amino-3-Hydroxy-5-Methylisoxazole-4-Propionic Acid (AMPA) Receptor Antagonist, on the Micturition Reflex in the Rat

Mitsuharu Yoshiyama, James R. Roppolo and William C. de Groat
Journal of Pharmacology and Experimental Therapeutics February 1, 1997, 280 (2) 894-904;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
OtherAUTONOMIC PHARMACOLOGY

Effects of LY215490, a Competitive α-Amino-3-Hydroxy-5-Methylisoxazole-4-Propionic Acid (AMPA) Receptor Antagonist, on the Micturition Reflex in the Rat

Mitsuharu Yoshiyama, James R. Roppolo and William C. de Groat
Journal of Pharmacology and Experimental Therapeutics February 1, 1997, 280 (2) 894-904;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • The Contribution of Classical (β1/2-) and Atypical β-Adrenoceptors to the Stimulation of Human White Adipocyte Lipolysis and Right Atrial Appendage Contraction by Novel β3-Adrenoceptor Agonists of Differing Selectivities
  • Mechanism of Gallbladder Relaxation in the Cat: Role of Norepinephrine ,
  • Gender Differences in the Expression of Endothelin Receptors in Human Saphenous Veins In Vitro
Show more AUTONOMIC PHARMACOLOGY

Similar Articles

  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2019 by the American Society for Pharmacology and Experimental Therapeutics