Protein kinase A Mediated Effects of Protein kinase C Partial Agonist HMI-1a3 in Colorectal Cancer Cells

Data Supplement

• Supplemental Data -

  Table S1. Necrotic cell death in Caco2 cells after 24-h exposure to the test compounds, measured by LDH assay.

  Table S2. Necrotic cell death in Colo205 cells after 24-h exposure to the test compounds, measured by LDH assay.

  Table S3. Necrotic cell death in HT29 cells after 24-h exposure to the test compounds, measured by LDH assay. Results are presented as mean (%) of cytotoxicity (N=3).

  Figure S1. HMI-1a3 induces apoptosis in all tested CRC lines.

  Figure S2. E2F1 and p21 expression in Caco2 (A), Colo205 (B) and HT29 (C) cells after 48-h exposure.

  Figure S3. Kinase tree after 30-minute HMI-1a3 20μM treatment on Colo205 cell lysates versus DMSO treated cell lysates.

  Figure S4. Kinase tree after 2-hour HMI-1a3 20μM treatment on Colo205 cell lysates versus DMSO treated cell lysates.