Modulation of actin affinity and actomyosin adenosine triphosphatase by charge changes in the myosin motor domain
M Furch, MA Geeves, DJ Manstein - Biochemistry, 1998 - ACS Publications
The effects of mutations in an actin-binding surface loop of myosin (loop 2) are described.
Part of loop 2, the segment between myosin residues 618 and 622, was replaced with …
Part of loop 2, the segment between myosin residues 618 and 622, was replaced with …
Single-molecule tracking of myosins with genetically engineered amplifier domains
C Ruff, M Furch, B Brenner, DJ Manstein… - Nature structural …, 2001 - nature.com
We combined protein engineering and single molecule measurements to directly record the
step size of a series of myosin constructs with shortened and elongated artificial neck …
step size of a series of myosin constructs with shortened and elongated artificial neck …
[HTML][HTML] Spray-dried lactose-leucine microparticles for pulmonary delivery of antimycobacterial nanopharmaceuticals
…, C Feldmann, C Hozsa, M Furch… - Drug delivery and …, 2021 - Springer
Pulmonary delivery of nanocarriers for novel antimycobacterial compounds is challenging
because the aerodynamic properties of nanomaterials are sub-optimal for such purposes. …
because the aerodynamic properties of nanomaterials are sub-optimal for such purposes. …
Role of the salt-bridge between switch-1 and switch-2 of Dictyostelium myosin
M Furch, S Fujita-Becker, MA Geeves… - Journal of molecular …, 1999 - Elsevier
Motifs N2 and N3, also referred to as switch-1 and switch-2, form part of the active site of
molecular motors such as myosins and kinesins. In the case of myosin, N3 is thought to act as a …
molecular motors such as myosins and kinesins. In the case of myosin, N3 is thought to act as a …
Antigen presenting cell-selective drug delivery by glycan-decorated nanocarriers
…, V Durán, C Hozsa, A Stępczyńska, M Furch… - European Journal of …, 2015 - Elsevier
Targeted drug delivery systems hold promise for selective provision of active compounds to
distinct tissues or cell subsets. Thus, locally enhanced drug concentrations are obtained that …
distinct tissues or cell subsets. Thus, locally enhanced drug concentrations are obtained that …
Nano‐in‐microparticles for aerosol delivery of antibiotic‐loaded, fucose‐derivatized, and macrophage‐targeted liposomes to combat mycobacterial infections: In Vitro …
…, C Hozsa, RK Gieseler, M Furch… - Advanced …, 2022 - Wiley Online Library
Nontuberculous mycobacterial infections rapidly emerge and demand potent medications to
cope with resistance. In this context, targeted loco‐regional delivery of aerosol medicines to …
cope with resistance. In this context, targeted loco‐regional delivery of aerosol medicines to …
[HTML][HTML] Simplified 89Zr-Labeling Protocol of Oxine (8-Hydroxyquinoline) Enabling Prolonged Tracking of Liposome-Based Nanomedicines and Cells
…, RR Pannem, F Manstein, T Borcholte, M Furch… - Pharmaceutics, 2021 - mdpi.com
In this work, a method for the preparation of the highly lipophilic labeling synthon [ 89 Zr]Zr(oxinate)
4 was optimized for the radiolabeling of liposomes and human induced pluripotent …
4 was optimized for the radiolabeling of liposomes and human induced pluripotent …
Stabilization of the actomyosin complex by negative charges on myosin
M Furch, B Remmel, MA Geeves, DJ Manstein - Biochemistry, 2000 - ACS Publications
Sequence comparisons of members of the myosin superfamily show a high degree of charge
conservation in a surface exposed helix (Dictyostelium discoideum myosin II heavy chain …
conservation in a surface exposed helix (Dictyostelium discoideum myosin II heavy chain …
Functional characterization of the secondary actin binding site of myosin II
J Van Dijk, M Furch, C Lafont, DJ Manstein… - Biochemistry, 1999 - ACS Publications
The role of the interaction between actin and the secondary actin binding site of myosin (segment
565−579 of rabbit skeletal muscle myosin, referred to as loop 3 in this work) has been …
565−579 of rabbit skeletal muscle myosin, referred to as loop 3 in this work) has been …
Charge changes in loop 2 affect the thermal unfolding of the myosin motor domain bound to F-actin
MA Ponomarev, M Furch, DI Levitsky, DJ Manstein - Biochemistry, 2000 - ACS Publications
The thermal unfolding of Dictyostelium discoideum myosin head fragments with alterations
in the actin-binding surface loop 2 was studied by differential scanning calorimetry. …
in the actin-binding surface loop 2 was studied by differential scanning calorimetry. …