Heterotrimeric guanine nucleotide–binding protein (G protein)–coupled receptors (GPCRs)
respond to a variety of different external stimuli and activate G proteins. GPCRs share many …

Background: Hepatitis C virus (HCV) is the major etiological agent of hepatocellular carcinoma,
and HCV RNA-dependent RNA polymerase (RdRp) is one of the main potential targets …

Mutations in MECP2 are associated with Rett syndrome, an X-linked neurodevelopmental
disorder. To identify genes targeted by Mecp2, we sequenced 100 in vivo Mecp2-binding sites …

… M NaCl buffer containing Complete Mini (Roche) protease inhibitor tablets. Extracts were
diluted to 0.1 M … The wild-type BUR column was washed with 0.1 M NaCl buffer before elution …

Hematopoietic prostaglandin (PG) D synthase is the key enzyme for production of the D and
J series of prostanoids in the immune system and mast cells. We isolated a cDNA for the rat …

… m Tris-HCl (pH 7.4), 5 mm dithiothreitol, 1.6 mm Triton X-100, 7.5 mm ATP, 10 mm MgCl 2
, 1 mm CoA, 0.2 mm potassium phosphoenolpyruvate, 0.15 mm NADH, 20 μg/ml adenylate …

Aging is driven by unavoidable entropic forces, physicochemical in nature, that damage the
raw materials that constitute biological systems. Single cells experience and respond to …

Photosystem II (PSII) catalyzes light-induced water splitting, leading to the evolution of
molecular oxygen indispensible for life on the earth. The crystal structure of PSII from …

… In addition, mutations of Asn 55, Tyr 59 and Tyr 97 did not affect the enzyme’s activity but
substantially increased its K m for GSH. The crystal structure reveals that these residues …

… Membrane fractions of HEK293 cells transfected with human BLT2 exhibited LTB 4 binding
with a Kd of 22.7 nm, ∼20-fold higher than that of BLT1 transfectants. BLT2 coupled to both …