Discovery of BRL 50481 [3-(N, N-dimethylsulfonamido)-4-methyl-nitrobenzene], a selective inhibitor of phosphodiesterase 7: in vitro studies in human monocytes, lung …
SJ Smith, LB Cieslinski, R Newton, LE Donnelly… - Molecular …, 2004 - ASPET
The biochemical and pharmacological characteristics in human proinflammatory cells of
BRL 50481 [3-(N,N-dimethylsulfonamido)-4-methyl-nitrobenzene], a novel and selective …
BRL 50481 [3-(N,N-dimethylsulfonamido)-4-methyl-nitrobenzene], a novel and selective …
Suppression of human inflammatory cell function by subtype‐selective PDE4 inhibitors correlates with inhibition of PDE4A and PDE4B
…, SB Christensen, LB Cieslinski… - British journal of …, 1999 - Wiley Online Library
Of the four major phosphodiesterase 4 (PDE4) subtypes, PDE4A, PDE4B and PDE4D are
widely expressed in human inflammatory cells, including monocytes and T lymphocytes. We …
widely expressed in human inflammatory cells, including monocytes and T lymphocytes. We …
Identification, characterization and functional role of phosphodiesterase isozymes in human airway smooth muscle.
TJ Torphy, BJ Undem, LB Cieslinski… - … of Pharmacology and …, 1993 - ASPET
In the present study phosphodiesterase (PDE) isozymes in human airway smooth muscle
were isolated, identified and characterized, and the functional roles of these isozymes in intact …
were isolated, identified and characterized, and the functional roles of these isozymes in intact …
1, 4-Cyclohexanecarboxylates: potent and selective inhibitors of phosophodiesterase 4 for the treatment of asthma
…, DE Griswold, LB Cieslinski… - Journal of medicinal …, 1998 - ACS Publications
… The systematic absences, 0k0 for k odd and h0l for l odd, indicated space group P2 1 /c.
Intensity data were collected on the diffractometer using variable speed ω−2θ scans where the …
Intensity data were collected on the diffractometer using variable speed ω−2θ scans where the …
A Candida albicans cyclic nucleotide phosphodiesterase: cloning and expression in Saccharomyces cerevisiae and biochemical characterization of the recombinant …
LL Hoyer, LB Cieslinski, MM McLaughlin… - …, 1994 - microbiologyresearch.org
We have cloned a Candida albicans gene, which encodes a cyclic nucleotide
phosphodiesterase (PDEase), by complementation in a Saccharomyces cerevisiae PDEase-deficient …
phosphodiesterase (PDEase), by complementation in a Saccharomyces cerevisiae PDEase-deficient …
[HTML][HTML] A low-Km, rolipram-sensitive, cAMP-specific phosphodiesterase from human brain. Cloning and expression of cDNA, biochemical characterization of …
MM McLaughlin, LB Cieslinski, M Burman… - Journal of Biological …, 1993 - Elsevier
We have isolated cDNA clones from human frontal cortex cDNA libraries that encode a
unique subtype of the low-Km, cAMP-specific phosphodiesterases (PDEs IV). The 564-amino …
unique subtype of the low-Km, cAMP-specific phosphodiesterases (PDEs IV). The 564-amino …
PDE4 inhibitors attenuate fibroblast chemotaxis and contraction of native collagen gels
…, HJ Kim, H Takizawa, LB Cieslinski… - American journal of …, 2002 - atsjournals.org
… 0.1 M TES (pH 8.0) followed by elution of the 5′-nucleotide product with 2 ml 2 M NaOH
into a scintillation vial containing 15 ml of Gold Ultima XR liquid scintillation cocktail (Sigma L-…
into a scintillation vial containing 15 ml of Gold Ultima XR liquid scintillation cocktail (Sigma L-…
Crystal structure, absolute configuration, and phosphodiesterase inhibitory activity of (+)-1-(4-bromobenzyl)-4-[(3-cyclopentyloxy)-4-methoxyphenyl]-2-pyrrolidinone
…, DS Eggleston, KF Erhard, LB Cieslinski… - Journal of medicinal …, 1993 - ACS Publications
… In the present study, the crystal structure of the l-(4-bromobenzyl) derivative of (-)-rolipram[(+)2]
is revealed, allowing the absolute configuration of (-)-l to be deduced. The PDE inhibitory …
is revealed, allowing the absolute configuration of (-)-l to be deduced. The PDE inhibitory …
Association of the anti-inflammatory activity of phosphodiesterase 4 (PDE4) inhibitors with either inhibition of PDE4 catalytic activity or competition for [3H] rolipram …
…, M Burman, SB Christensen, LB Cieslinski… - Biochemical …, 1996 - Elsevier
… phosphodiesterase (PDE4) have demonstrated impressive in vitro anti-inflammatory actions
[l-7]. The promise that these inhibitors may be useful anti-inflammatory agents is tempered …
[l-7]. The promise that these inhibitors may be useful anti-inflammatory agents is tempered …
Characterization and selective inhibition of cyclic nucleotide phosphodiesterase isozymes in canine tracheal smooth muscle.
TJ Torphy, LB Cieslinski - Molecular Pharmacology, 1990 - ASPET
Cyclic nucleotide phosphodiesterases (PDEs) from canine trachealis were characterized
with respect to their kinetic properties, sensitivity to selective inhibitors, and subcellular …
with respect to their kinetic properties, sensitivity to selective inhibitors, and subcellular …