… High computational efficiency of PPM 2.0 allows its application for the large-scale
analysis of proteins from the PDB. The results are deposited in our Orientation of Proteins in …

… the general lack of enkephalin analogs witha high degree of selectivity for a single receptor
… receptor (4-6) anda nonpeptide opiate with high K receptor selectivity (7). However, analogs …

… and HI Mosberg, manusript submitted). The developed theoretical approach discriminates
between TM and water-soluble proteins and determines the positions of TM proteins with a …

The opioid receptors involved in the mediation of thermal analgesia (55 degrees C hot-plate)
and inhibition of gastrointestinal transit at the spinal and supraspinal levels were studied in …

Opioid receptors interact with a variety of ligands, including endogenous peptides, opiates,
and thousands of synthetic compounds with different structural scaffolds. In the absence of …

… Only residues located in regions of sufficiently high polarity could produce the reactive
thiolate anion required for the formation of intermolecular disulfide bonds. Indeed, our …

… ]DPN binding with a Ki hi of ∼2.9 nm and a Ki lo of ∼1.5 μm (fraction of Ki hi ∼0.53). The
addition of 10 μm GTPγS to the YMOR + Gi 2 membranes eliminated the high affinity DAMGO-…

… The solution was dried under a stream of nitrogen and further dried under high vacuum for
several hours. Buffer (10 mM Tris/HCl, 100 mM NaCl, 2 mM EDTA, pH 7.4) was added to each …

… However, the significant advantage of our method is its high computational efficiency and
its ability to operate directly with transfer free energies that can be easily compared with …

Methods Subjects. Male, Harlan ND4 mice (Indianapolis, IN) weighing between 18 and 24 g
were housed in groups of eight per cage in a temperature-controlled room maintained on a …