… In this cyclase, substrate ionization is achieved by the protonation of a C=C double bond (Figure
5a). Site-directed mutagenesis studies [23] interpreted in conjunction with the crystal …

INTRODUCTION Activation of the STING (stimulator of interferon genes) protein by its natural
ligand, cyclic guanosine monophosphate–adenosine monophosphate (cGAMP), triggers …

… The structural diversity and stereochemical complexity of the C 15 -isoprenoid skeletons
of these metabolites are remarkable. Indeed, of the more than 300 cyclic sesquiterpenes that …

Various classes of nucleotidyl polymerases with different transcriptional roles contain a
conserved core structure. Less is known, however, about the distinguishing features of these …

… at 16 C at 250 rpm with 85% RH. Cells were harvested by centrifugation at 4,000 rpm at 4 C
for 20 min and the media discarded. The cell pellets were stored at −80 C until ready for use. …

… lid residues such as Gly-230 and Gly-234 (Figure 2C, right), as well as several other regions
in the spectra (Figure 2C, left, for example), consistent with the pronounced changes in the …

… Optical absorption spectra were collected on 60 μM Co 2+ -E117Q CAII in 10 mM buffer (Tris,
pH 8.0, and CHES, pH 9.1 and 9.9) at 25 C with the same concentration of Zn 2+ -enzyme …

Drugging large protein pockets is a challenge due to the need for higher molecular weight
ligands, which generally possess undesirable physicochemical properties. In this …

… 67Similarly, D235 in cytochrome c peroxidase, which forms a hydrogen bond to proximal …
,7 The neutralization of side chain charge in D235N cytochrome c peroxidase results in a 5-fold …

… calculated using the Kaleidagraph 3.0 (Synergy Software) curve-fitting program with eq 4
where C ranged from 0.9 to 1.1. … c K D for dansylamide measured in 50 mM MOPS, pH 7.9, 25 …