Abstract
A cDNA encoding an organic cation transporter (rbOCT1) was isolated from rabbit kidney. The cDNA encodes a 554 amino acid protein that is highly homologous to other mammalian organic cation transporters. rbOCT1 mediated 3H-1-methyl-4-phenylpyridinium (3H-MPP+) transport in Xenopus laevis oocytes was saturable, sensitive to membrane potential, and inhibited by various organic cations. rbOCT1 mRNA transcripts are expressed in the kidney, liver, and intestine.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Carrier Proteins / genetics*
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Cloning, Molecular
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DNA, Complementary
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Kidney / metabolism*
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Male
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Membrane Proteins / genetics*
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Molecular Sequence Data
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Organic Cation Transporter 1
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Polymerase Chain Reaction
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RNA, Messenger / genetics
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Rabbits
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Xenopus laevis
Substances
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Carrier Proteins
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DNA, Complementary
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Membrane Proteins
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Organic Cation Transporter 1
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RNA, Messenger