Abstract
Expression of Shc family protein (Shc/ShcA, SCK/ShcB and N-Shc/ShcC) and Grb2 mRNAs in the hypoglossal motoneurons after axotomy was examined by in situ hybridization. In normal hypoglossal motor neurons, N-Shc mRNA was expressed predominantly, whereas the Shc mRNA level is very low. Rat hypoglossal nerve injury reversed the expressions of these two molecules in hypoglossal motoneurons. Shc mRNA expression was up-regulated markedly whereas N-Shc was down-regulated after nerve injury. Expression levels of SCK, another Shc family member, and Grb2 were unaffected by nerve injury. These results suggest that, whereas the N-Shc-mediated pathway dominates under normal conditions, an alternative Shc-mediated pathway is utilized in the event of nerve injury. By changing the expression of the Shc family members, the signaling pathway can be altered and various responses induced for nerve regeneration.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Adaptor Proteins, Vesicular Transport*
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Animals
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GRB2 Adaptor Protein
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Gene Expression Regulation
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Hypoglossal Nerve / metabolism*
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Hypoglossal Nerve Injuries*
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Male
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Motor Neurons / metabolism*
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Protein Biosynthesis*
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Proteins*
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RNA Probes
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RNA, Messenger / biosynthesis
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Rats
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Rats, Wistar
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Shc Signaling Adaptor Proteins
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Src Homology 2 Domain-Containing, Transforming Protein 1
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Src Homology 2 Domain-Containing, Transforming Protein 2
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Time Factors
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Transcription, Genetic*
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src Homology Domains
Substances
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport
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GRB2 Adaptor Protein
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Grb2 protein, rat
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Proteins
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RNA Probes
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RNA, Messenger
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Shc Signaling Adaptor Proteins
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Shc1 protein, rat
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Shc2 protein, rat
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Src Homology 2 Domain-Containing, Transforming Protein 1
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Src Homology 2 Domain-Containing, Transforming Protein 2