Abstract
The convulsive agent pentylenetetrazol (PTZ) antagonized gamma-aminobutyric acid (GABA)-induced membrane currents on RNA-injected Xenopus oocytes with both extra- and intracellular applications. With extracellular administration PTZ enters the cell within a few minutes and reaches concentrations in the millimolar range. The permeability of the plasma membrane makes it possible for systemically applied PTZ to elicit its effect on the GABA-induced currents via extra- as well as intracellular sites of action.
MeSH terms
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Animals
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Cell Membrane / metabolism
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Convulsants / pharmacology*
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Female
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GABA Antagonists / pharmacology*
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Ganglionic Stimulants / pharmacology
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Membrane Potentials / drug effects
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Microelectrodes
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Microinjections
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Oocytes / drug effects*
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Oocytes / ultrastructure
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Patch-Clamp Techniques
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Pentylenetetrazole / pharmacokinetics
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Pentylenetetrazole / pharmacology*
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Quaternary Ammonium Compounds / pharmacology
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RNA / pharmacology
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Rats
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Receptors, GABA / physiology*
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Xenopus laevis
Substances
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Convulsants
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GABA Antagonists
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Ganglionic Stimulants
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Quaternary Ammonium Compounds
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Receptors, GABA
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RNA
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tetramethylammonium
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Pentylenetetrazole