Abstract
The addition of cAMP inhibits G-CSF-mediated proliferation and suppresses pRB phosphorylation in NFS-60 cells. We show that the latter could be attributed to different effects of cAMP in these cells: (i) down-regulation of the levels of cyclins D2 and D3, and cdk4, and (ii) induction of the p27Kip1 inhibitor of cdk4.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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1-Methyl-3-isobutylxanthine / pharmacology
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8-Bromo Cyclic Adenosine Monophosphate / analogs & derivatives
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology*
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Animals
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Blotting, Northern
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Cell Cycle Proteins*
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Cell Division / drug effects
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Cell Line
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Cyclic AMP / analogs & derivatives
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Cyclic AMP / pharmacology*
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Cyclin D
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinase Inhibitor p27
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Cyclin-Dependent Kinases / biosynthesis*
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Cyclins / biosynthesis*
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DNA / biosynthesis
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Dinoprostone / pharmacology
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Dose-Response Relationship, Drug
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Genes, Tumor Suppressor
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Granulocyte Colony-Stimulating Factor / pharmacology*
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Kinetics
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Microtubule-Associated Proteins / biosynthesis*
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Phosphorylation
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Proto-Oncogene Proteins*
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Tumor Suppressor Proteins*
Substances
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Cell Cycle Proteins
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Cyclin D
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Cyclins
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Microtubule-Associated Proteins
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Proto-Oncogene Proteins
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Tumor Suppressor Proteins
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Granulocyte Colony-Stimulating Factor
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Cyclin-Dependent Kinase Inhibitor p27
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8-Bromo Cyclic Adenosine Monophosphate
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DNA
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Cyclic AMP
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases
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Dinoprostone
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1-Methyl-3-isobutylxanthine