Abstract
We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The receptor encoded by GPR9 shares the highest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% overall and 51% in the transmembrane domains). GPR10 encodes a receptor that shares highest identity with the neuropeptide Y receptor (31% overall and 46% in the transmembrane domains). The receptor encoded by GPR14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hybridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Antigens, CD / biosynthesis
-
Antigens, CD / chemistry
-
Antigens, CD / genetics*
-
Base Sequence
-
Blotting, Northern
-
Brain / metabolism
-
Chromosome Mapping
-
Chromosomes, Human, Pair 8*
-
Cloning, Molecular
-
DNA
-
Gene Library
-
Humans
-
In Situ Hybridization, Fluorescence
-
Interleukin-8 / metabolism
-
Lymphocytes / cytology
-
Lymphocytes / immunology
-
Lymphocytes / metabolism
-
Molecular Sequence Data
-
Polymerase Chain Reaction
-
RNA, Messenger / isolation & purification
-
RNA, Messenger / metabolism
-
Receptors, Interleukin / biosynthesis
-
Receptors, Interleukin / chemistry
-
Receptors, Interleukin / genetics*
-
Receptors, Interleukin-8A
-
Receptors, Neuropeptide Y / biosynthesis
-
Receptors, Neuropeptide Y / chemistry
-
Receptors, Neuropeptide Y / genetics*
-
Receptors, Opioid / chemistry
-
Receptors, Opioid / genetics
-
Receptors, Somatostatin / biosynthesis
-
Receptors, Somatostatin / chemistry
-
Receptors, Somatostatin / genetics*
-
Recombinant Proteins / biosynthesis
-
Recombinant Proteins / chemistry
-
Sequence Homology, Amino Acid
Substances
-
Antigens, CD
-
Interleukin-8
-
RNA, Messenger
-
Receptors, Interleukin
-
Receptors, Interleukin-8A
-
Receptors, Neuropeptide Y
-
Receptors, Opioid
-
Receptors, Somatostatin
-
Recombinant Proteins
-
DNA
Associated data
-
GENBANK/U32672
-
GENBANK/U32673
-
GENBANK/U32674