Cloning and chromosomal mapping of three novel genes, GPR9, GPR10, and GPR14, encoding receptors related to interleukin 8, neuropeptide Y, and somatostatin receptors

Genomics. 1995 Sep 20;29(2):335-44. doi: 10.1006/geno.1995.9996.

Abstract

We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The receptor encoded by GPR9 shares the highest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% overall and 51% in the transmembrane domains). GPR10 encodes a receptor that shares highest identity with the neuropeptide Y receptor (31% overall and 46% in the transmembrane domains). The receptor encoded by GPR14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hybridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antigens, CD / biosynthesis
  • Antigens, CD / chemistry
  • Antigens, CD / genetics*
  • Base Sequence
  • Blotting, Northern
  • Brain / metabolism
  • Chromosome Mapping
  • Chromosomes, Human, Pair 8*
  • Cloning, Molecular
  • DNA
  • Gene Library
  • Humans
  • In Situ Hybridization, Fluorescence
  • Interleukin-8 / metabolism
  • Lymphocytes / cytology
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism
  • Receptors, Interleukin / biosynthesis
  • Receptors, Interleukin / chemistry
  • Receptors, Interleukin / genetics*
  • Receptors, Interleukin-8A
  • Receptors, Neuropeptide Y / biosynthesis
  • Receptors, Neuropeptide Y / chemistry
  • Receptors, Neuropeptide Y / genetics*
  • Receptors, Opioid / chemistry
  • Receptors, Opioid / genetics
  • Receptors, Somatostatin / biosynthesis
  • Receptors, Somatostatin / chemistry
  • Receptors, Somatostatin / genetics*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Homology, Amino Acid

Substances

  • Antigens, CD
  • Interleukin-8
  • RNA, Messenger
  • Receptors, Interleukin
  • Receptors, Interleukin-8A
  • Receptors, Neuropeptide Y
  • Receptors, Opioid
  • Receptors, Somatostatin
  • Recombinant Proteins
  • DNA

Associated data

  • GENBANK/U32672
  • GENBANK/U32673
  • GENBANK/U32674