Abstract
To characterize the alpha1-adrenoceptor subtypes, we developed a flow cytometry method using the fluorescent ligand BODIPY-FL prazosin and the anti-peptide antibody against the alpha1b-adrenoceptor amino terminus (designated 1B-N1-C) as probes. Three alpha1-adrenoceptors (alpha1a, alpha1b and alpha1d) expressed in CHO cells were detected by BODIPY-FL prazosin; however, only alpha1b-adrenoceptor subtype was detected by the anti-peptide antibody 1B-N1-C. Furthermore, the flow cytometry analysis with 1B-N1-C specifically identified alpha1b-adrenoceptor in native cells of hamster DDT1-MF2 cells, rat hepatocytes and cardiomyocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Boron Compounds / chemistry
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CHO Cells
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Cells, Cultured
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Chlorocebus aethiops
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Cricetinae
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Flow Cytometry / methods*
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Fluorescent Dyes / chemistry
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Humans
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Liver / cytology
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Molecular Sequence Data
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Molecular Structure
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Myocardium / cytology
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Rabbits
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Rats
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Receptors, Adrenergic, alpha-1 / analysis*
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Receptors, Adrenergic, alpha-1 / classification
Substances
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4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
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ADRA1A protein, human
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ADRA1B protein, human
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ADRA1D protein, human
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Adra1a protein, rat
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Adra1b protein, rat
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Adra1d protein, rat
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Boron Compounds
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Fluorescent Dyes
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Receptors, Adrenergic, alpha-1