Abstract
Inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ signaling plays important roles in cellular responses to extracellular stimuli. We recently succeeded in cloning human counterparts of the three subtypes derived from separate genes. Using the cDNA sequences type-specific to these subtype receptors, we here analyzed the expression profile of IP3R subtypes in stimulated and unstimulated human hematopoietic cell lines representing T cells, B cells, neutrophils, macrophages, erythrocytes and megakaryocytes. Northern and dot blot analysis showed that each IP3R subtype is expressed differently in these cells and that the expression profile in each cell is dynamically changed upon stimuli which induce differentiation. Moreover, most of these cells were found to simultaneously express at least two different subtype receptors.
MeSH terms
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B-Lymphocytes / cytology
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B-Lymphocytes / metabolism
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Blotting, Northern
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Calcium Channels / genetics
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Calcium Channels / metabolism*
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Cell Differentiation
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Cell Line
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Cloning, Molecular
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Erythrocytes / cytology
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Erythrocytes / metabolism
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Hematopoietic Stem Cells / cytology
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Hematopoietic Stem Cells / metabolism*
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Humans
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Inositol 1,4,5-Trisphosphate / metabolism*
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Inositol 1,4,5-Trisphosphate Receptors
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Macrophages / cytology
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Macrophages / metabolism
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Megakaryocytes / cytology
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Megakaryocytes / metabolism
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Neutrophils / cytology
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Neutrophils / metabolism
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Organ Specificity / genetics
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RNA, Messenger / isolation & purification
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / metabolism*
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T-Lymphocytes / cytology
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T-Lymphocytes / metabolism
Substances
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Calcium Channels
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ITPR1 protein, human
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Inositol 1,4,5-Trisphosphate Receptors
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RNA, Messenger
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Receptors, Cytoplasmic and Nuclear
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Inositol 1,4,5-Trisphosphate