Molecular cloning of rat cDNA for cytosolic phospholipase A2 and the increased gene expression in the dentate gyrus following transient forebrain ischemia

Brain Res Mol Brain Res. 1994 Sep;25(3-4):364-8. doi: 10.1016/0169-328x(94)90174-0.

Abstract

Rat cytosolic phospholipase A2 (cPLA2) cDNA was cloned from rat brain. The cDNA showed a high homology of 90% in the nucleotide sequence of the coding region with the human counterpart. By in situ hybridization, the gene expression for cPLA2 was detected in the hippocampus, olfactory bulb, and cerebellar granular cells at very low level of normal rats. The expression was markedly increased in the dentate granule cells of postischemic rat brain. This alteration of the gene expression was discussed in relation to the free fatty acid-mediated neurotoxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Cytosol / enzymology*
  • DNA, Complementary / isolation & purification*
  • Gene Expression
  • Hippocampus / metabolism*
  • Ischemic Attack, Transient / metabolism*
  • Male
  • Molecular Sequence Data
  • Phospholipases A / genetics*
  • Phospholipases A2
  • Prosencephalon / blood supply*
  • Rats
  • Rats, Wistar

Substances

  • DNA, Complementary
  • Phospholipases A
  • Phospholipases A2