Molecular cloning and expression of a delta-opioid receptor from rat brain

J Neurosci Res. 1994 Apr 15;37(6):714-9. doi: 10.1002/jnr.490370605.

Abstract

We have isolated and characterized a rat delta-opioid receptor. The deduced amino acid sequence (372 aa) closely resembles the murine delta-opioid receptor, DOR-1. In fact, 97% of the amino acid residues are conserved between the two species, while 93% of the nucleic acid residues are identical. A 6 kb mRNA was detected in rat cortex using rat DOR-1 as a probe. When expressed in COS cells, the clone shows high-affinity opioid binding with selectivity for delta-opioids. The rat delta-opioid receptor cDNA clone will be a useful tool for studying the function of delta-opioid receptor in rats.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Brain Chemistry / physiology*
  • Cells, Cultured
  • Cloning, Molecular
  • DNA Probes
  • DNA, Complementary / biosynthesis
  • Diprenorphine / pharmacokinetics
  • Gene Library
  • Molecular Sequence Data
  • Olfactory Bulb / metabolism
  • RNA / analysis
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Opioid, delta / biosynthesis*
  • Receptors, Opioid, delta / genetics
  • Transfection

Substances

  • DNA Probes
  • DNA, Complementary
  • Receptors, Opioid, delta
  • Diprenorphine
  • RNA

Associated data

  • GENBANK/U00475