Abstract
(-)-Naloxone, 1 mM, partially reduced neuronal loss induced by exposure of murine cortical cell cultures to N-methyl-D-aspartate (NMDA) or quinolinate, but produced little or no attenuation of kainate or quisqualate neurotoxicity. Antagonism of NMDA neurotoxicity was (-)-naloxone concentration-dependent between 100 microM and 3 mM. (+)-Naloxone produced a slightly greater reduction of NMDA neurotoxicity, arguing against mediation by opioid receptors. Although this novel neuron-protective action of (-)-naloxone was weak, it may contribute to reported beneficial effects in CNS ischemia.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Aspartic Acid / analogs & derivatives
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Aspartic Acid / toxicity
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Cells, Cultured
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Cerebral Cortex / cytology
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Female
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L-Lactate Dehydrogenase / metabolism
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Mice
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N-Methylaspartate
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Naloxone / pharmacology*
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Nervous System Diseases / chemically induced*
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Nervous System Diseases / enzymology
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Oxadiazoles / toxicity
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Pregnancy
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Quisqualic Acid
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Receptors, N-Methyl-D-Aspartate
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Receptors, Neurotransmitter / physiology*
Substances
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Oxadiazoles
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Receptors, N-Methyl-D-Aspartate
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Receptors, Neurotransmitter
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Aspartic Acid
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Naloxone
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N-Methylaspartate
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Quisqualic Acid
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L-Lactate Dehydrogenase