Action of calcitonin gene-related peptide on rat aortic smooth muscle

Eur J Pharmacol. 1989 Jul 18;166(2):219-22. doi: 10.1016/0014-2999(89)90062-9.

Abstract

The effects of rat calcitonin gene-related peptide (CGRP) on superfused rat aortic smooth muscle cells in cell culture were investigated. Exposure of the cells for 10 min to CGRP (10(-7) M), with or without pretreatment with pertussis toxin, stimulated the release of cyclic AMP but not of prostacyclin, as judged by radioimmunoassay of its stable metabolite, 6-keto-PGF1 alpha. Pretreatment of the cells with pertussis toxin did not alter the response to CGRP. The direct action of CGRP on smooth muscle cells and on the CGRP-induced formation of cyclic AMP did not appear to depend on the production of prostacyclin in these vascular smooth muscle cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 6-Ketoprostaglandin F1 alpha / biosynthesis
  • Angiotensin II / pharmacology
  • Animals
  • Aorta, Thoracic / drug effects
  • Aorta, Thoracic / metabolism
  • Arginine Vasopressin / pharmacology
  • Calcitonin Gene-Related Peptide / pharmacology*
  • Cells, Cultured
  • Colforsin / pharmacology
  • Cyclic AMP / biosynthesis
  • Dinoprost / biosynthesis
  • Dinoprostone / biosynthesis
  • In Vitro Techniques
  • Muscle, Smooth, Vascular / drug effects*
  • Muscle, Smooth, Vascular / metabolism
  • Rats

Substances

  • Angiotensin II
  • Arginine Vasopressin
  • Colforsin
  • 6-Ketoprostaglandin F1 alpha
  • Dinoprost
  • Cyclic AMP
  • Calcitonin Gene-Related Peptide
  • Dinoprostone