Abstract
The effects of rat calcitonin gene-related peptide (CGRP) on superfused rat aortic smooth muscle cells in cell culture were investigated. Exposure of the cells for 10 min to CGRP (10(-7) M), with or without pretreatment with pertussis toxin, stimulated the release of cyclic AMP but not of prostacyclin, as judged by radioimmunoassay of its stable metabolite, 6-keto-PGF1 alpha. Pretreatment of the cells with pertussis toxin did not alter the response to CGRP. The direct action of CGRP on smooth muscle cells and on the CGRP-induced formation of cyclic AMP did not appear to depend on the production of prostacyclin in these vascular smooth muscle cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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6-Ketoprostaglandin F1 alpha / biosynthesis
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Angiotensin II / pharmacology
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Animals
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Aorta, Thoracic / drug effects
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Aorta, Thoracic / metabolism
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Arginine Vasopressin / pharmacology
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Calcitonin Gene-Related Peptide / pharmacology*
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Cells, Cultured
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Colforsin / pharmacology
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Cyclic AMP / biosynthesis
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Dinoprost / biosynthesis
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Dinoprostone / biosynthesis
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In Vitro Techniques
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Muscle, Smooth, Vascular / drug effects*
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Muscle, Smooth, Vascular / metabolism
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Rats
Substances
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Angiotensin II
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Arginine Vasopressin
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Colforsin
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6-Ketoprostaglandin F1 alpha
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Dinoprost
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Cyclic AMP
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Calcitonin Gene-Related Peptide
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Dinoprostone