Ultrastructural localisation of substance P and choline acetyltransferase in endothelial cells of rat coronary artery and release of substance P and acetylcholine during hypoxia

P Milner; V Ralevic; A M Hopwood; E Fehér; J Lincoln; K A Kirkpatrick; G Burnstock

doi:10.1007/BF01954843

Ultrastructural localisation of substance P and choline acetyltransferase in endothelial cells of rat coronary artery and release of substance P and acetylcholine during hypoxia

Experientia. 1989 Feb 15;45(2):121-5. doi: 10.1007/BF01954843.

Authors

P Milner¹, V Ralevic, A M Hopwood, E Fehér, J Lincoln, K A Kirkpatrick, G Burnstock

Affiliation

¹ University College London, Department of Anatomy and Developmental Biology, United Kingdom.

PMID: 2465912
DOI: 10.1007/BF01954843

Abstract

Substance P and choline acetyltransferase have been localised in a small proportion of endothelial cells of rat coronary arteries using electron microscopic immunocytochemistry. During a hypoxic period of 1 min, coronary vasodilatation was produced in the Langendorff heart preparation and increased levels of substance P and acetylcholine were released into the perfusate. The possibility that these substances are released from endothelial cells during hypoxia and contribute to the hyperaemic response is discussed.

MeSH terms

Acetylcholine / metabolism*
Animals
Choline O-Acetyltransferase / analysis
Choline O-Acetyltransferase / metabolism*
Coronary Vessels / metabolism
Coronary Vessels / ultrastructure*
Endothelium, Vascular / drug effects
Endothelium, Vascular / metabolism
Endothelium, Vascular / ultrastructure*
Histocytochemistry
Immunoenzyme Techniques
Male
Microscopy, Electron
Oxygen / administration & dosage
Oxygen / pharmacology*
Rats
Rats, Inbred Strains
Substance P / analysis
Substance P / metabolism*

Substances

Substance P
Choline O-Acetyltransferase
Acetylcholine
Oxygen