Abstract
Degradation of a synthetic oligodeoxynucleoside phosphorothioate was studied in six systems used for antisense inhibition experiments. Oligodeoxynucleoside phosphorothioates were degraded very slowly at 37 degrees C in all of the systems studied. Measured half-lives of pentadecamers were 12 +/- 1 h in rabbit reticulocyte lysate, 7 +/- 1 h in HeLa cell postmitochondrial extract, 14 +/- 2 h in RPMI 1640 with 10% fetal bovine serum, 8 +/- 1 h in undiluted fetal bovine serum, 9 +/- 1 h in adult human serum, and 19 +/- 7 h in rat cerebrospinal fluid.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cell Extracts / analysis
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Culture Media
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HeLa Cells
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Humans
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Molecular Sequence Data
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Oligonucleotides / metabolism
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Oligonucleotides, Antisense
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-myc
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Rabbits
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Rats
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Reticulocytes / metabolism
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Thionucleotides / blood
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Thionucleotides / cerebrospinal fluid
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Thionucleotides / metabolism*
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Time Factors
Substances
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Cell Extracts
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Culture Media
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Oligonucleotides
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Oligonucleotides, Antisense
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-myc
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Thionucleotides