Immunological identification of the mouse H4 histamine receptor on spinal cord motor neurons using a novel anti-mouse H4R antibody

Inflamm Res. 2010 Mar:59 Suppl 2:S197-8. doi: 10.1007/s00011-009-0127-2.

Abstract

In this report, we describe the development of the first anti-mouse H(4) receptor antibody. Utilising this new immunological probe, new evidence is provided for oligomeric mH(4)Rs and the presence of H(4) receptors on a subpopulation of murine motor neurons. This expands the cell types where the H(4) receptor is expressed in the mammalian CNS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Blocking / pharmacology*
  • Antibody Specificity
  • Blotting, Western
  • Immunohistochemistry
  • Mice
  • Molecular Sequence Data
  • Motor Neurons / metabolism*
  • Receptors, G-Protein-Coupled / immunology
  • Receptors, G-Protein-Coupled / metabolism*
  • Receptors, Histamine / immunology
  • Receptors, Histamine / metabolism*
  • Receptors, Histamine H4
  • Spinal Cord / cytology
  • Spinal Cord / metabolism*
  • Transfection

Substances

  • Antibodies, Blocking
  • Hrh4 protein, mouse
  • Receptors, G-Protein-Coupled
  • Receptors, Histamine
  • Receptors, Histamine H4