Preparing serum for functional complement assays

J Immunol Methods. 2010 Jan 31;352(1-2):195-7. doi: 10.1016/j.jim.2009.11.003. Epub 2009 Nov 10.

Abstract

To conserve full complement activity, a number of precautions need to be taken in preparing serum. This is particularly the case with mouse complement where the classical pathway is notoriously unstable.

MeSH terms

  • Animals
  • Blood Proteins / immunology
  • Blood Proteins / metabolism*
  • Complement Pathway, Classical
  • Complement System Proteins / immunology
  • Complement System Proteins / metabolism*
  • Cytotoxicity Tests, Immunologic / methods*
  • Diagnostic Errors / prevention & control
  • Health Planning Guidelines
  • Mice
  • Protein Stability
  • Reproducibility of Results
  • Serum / metabolism
  • Specimen Handling

Substances

  • Blood Proteins
  • Complement System Proteins