Abstract
We utilized the technique of polymerase chain reaction with oligonucleotide primers based upon the nucleotide sequence of the canine H2 histamine receptor gene which we recently isolated to clone its human homologue. Transfection of a construct of this gene in Colo-320 DM cells led to the expression of a receptor that bound to [methyl-3H] tiotidine and was linked to 3',5'cyclic adenosine monophosphate (cAMP) generation in response to histamine. Both cAMP generation and [methyl-3H] tiotidine binding were inhibited with the H2 histamine receptor selective antagonist cimetidine but not diphenhydramine or thioperamide which are, respectively, H1 and H3 histamine receptor antagonists. These data confirm that we have successfully cloned a novel gene encoding the human H2 histamine receptor.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cell Line
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Cloning, Molecular / methods
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Colonic Neoplasms
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Dogs
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Gastric Mucosa / physiology
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Genetic Vectors
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Humans
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Kinetics
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Molecular Sequence Data
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Oligonucleotide Probes
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Poly A / genetics
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Poly A / isolation & purification
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Polymerase Chain Reaction
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger
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Receptors, Histamine H2 / genetics*
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Receptors, Histamine H2 / metabolism
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Sequence Homology, Nucleic Acid
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Transfection
Substances
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Oligonucleotide Probes
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RNA, Messenger
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Receptors, Histamine H2
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Poly A
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RNA
Associated data
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GENBANK/M64799
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GENBANK/S50206
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GENBANK/S50208
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GENBANK/S50210
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GENBANK/S50212
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GENBANK/S50216
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GENBANK/S50220
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GENBANK/S50222
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GENBANK/S50224
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GENBANK/S51292