Abstract
The inhibition of voltage-dependent Ca2+ channels in secretory cells by plasma membrane receptors is mediated by pertussis toxin-sensitive G proteins. Multiple forms of G proteins have been described, differing principally in their alpha subunits, but it has not been possible to establish which G-protein subtype mediates inhibition by a specific receptor. By intranuclear injection of antisense oligonucleotides into rat pituitary GH3 cells, the essential role of the Go-type G proteins in Ca(2+)-channel inhibition is established: the subtypes Go1 and Go2 mediate inhibition through the muscarinic and somatostatin receptors, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Calcium Channels / physiology*
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Carbachol / pharmacology
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Cell Line
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Cell Membrane / physiology
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DNA, Antisense / genetics
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Electric Conductivity
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Electrophysiology
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GTP-Binding Proteins / genetics
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GTP-Binding Proteins / physiology*
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Microinjections
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Molecular Sequence Data
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Pertussis Toxin
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Pituitary Gland / physiology
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RNA, Messenger / genetics
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Rats
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Receptors, Muscarinic / physiology*
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Receptors, Neurotransmitter / physiology*
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Receptors, Somatostatin
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Signal Transduction
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Somatostatin / pharmacology
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Virulence Factors, Bordetella / pharmacology
Substances
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Calcium Channels
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DNA, Antisense
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RNA, Messenger
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Receptors, Muscarinic
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Receptors, Neurotransmitter
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Receptors, Somatostatin
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Virulence Factors, Bordetella
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Somatostatin
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Carbachol
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Pertussis Toxin
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GTP-Binding Proteins