The effect of hypoxia on lipid phosphate receptor and sphingosine kinase expression and mitogen-activated protein kinase signaling in human pulmonary smooth muscle cells

Maqsood Ahmad; Jaclyn S Long; Nigel J Pyne; Susan Pyne

doi:10.1016/j.prostaglandins.2006.03.001

The effect of hypoxia on lipid phosphate receptor and sphingosine kinase expression and mitogen-activated protein kinase signaling in human pulmonary smooth muscle cells

Prostaglandins Other Lipid Mediat. 2006 May;79(3-4):278-86. doi: 10.1016/j.prostaglandins.2006.03.001. Epub 2006 Apr 18.

Authors

Maqsood Ahmad¹, Jaclyn S Long, Nigel J Pyne, Susan Pyne

Affiliation

¹ Department of Physiology and Pharmacology, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, G4 0NR, UK.

PMID: 16647641
DOI: 10.1016/j.prostaglandins.2006.03.001

Abstract

Both acute and chronic hypoxia had no effect on S1P(1), S1P(3) or LPA(1) receptor transcript expression in human pulmonary smooth muscle cells. However, acute hypoxia increased sphingosine kinase SK1/2 and LPP1 mRNA transcript levels, while chronic hypoxia increased SK1 mRNA transcript alone. Acute hypoxia had no effect on S1P-, PDGF- or phorbol ester (PMA)-stimulated activation of ERK-1/2, but increased the ability of S1P to activate p38 MAPK. Chronic hypoxia increased the ability of S1P to stimulate the phosphorylation of ERK-1/2. Therefore, we have demonstrated for the first time that hypoxia can lead to marked changes in the expression of genes involved in S1P production and may modify post S1P receptor signal transduction pathways.

MeSH terms

Cell Hypoxia
Cells, Cultured
Gene Expression Regulation / drug effects
Humans
Lung / cytology
Lung / enzymology
Lung / metabolism*
Lysophospholipids / genetics
Lysophospholipids / metabolism
MAP Kinase Signaling System*
Myocytes, Smooth Muscle / cytology
Myocytes, Smooth Muscle / enzymology
Myocytes, Smooth Muscle / metabolism*
Phosphatidate Phosphatase / genetics
Phosphatidate Phosphatase / metabolism
Phosphotransferases (Alcohol Group Acceptor) / genetics
Phosphotransferases (Alcohol Group Acceptor) / metabolism*
Receptors, Lysophosphatidic Acid / metabolism*
Receptors, Lysosphingolipid / metabolism*
Sphingosine / analogs & derivatives
Sphingosine / genetics
Sphingosine / metabolism

Substances

Lysophospholipids
Receptors, Lysophosphatidic Acid
Receptors, Lysosphingolipid
sphingosine 1-phosphate
Phosphotransferases (Alcohol Group Acceptor)
sphingosine kinase
lipid phosphate phosphatase
Phosphatidate Phosphatase
Sphingosine
lysophosphatidic acid