Abstract
cGMP kinase I (cGKI) signaling modulates multiple physiological processes including smooth muscle relaxation. The expression of cGKI and its substrate IRAG (Inositol 1,4,5-trisphosphate receptor associated cGMP kinase substrate) was studied. IRAG and cGKI were colocalized in the smooth muscle of aorta and colon. IRAG was present in the thalamus and in most of the myenteric plexus in the absence of cGKI. Coexpression of IRAG and cGKIbeta or cGKIalpha in COS-7 cells revealed that IRAG recruits cGKIbeta but not cGKIalpha to the endoplasmic reticulum. These results suggest that IRAG may be involved in cGKI-dependent and -independent pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Calcium Channels / metabolism
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Cyclic GMP-Dependent Protein Kinase Type I
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Cyclic GMP-Dependent Protein Kinases / genetics
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Cyclic GMP-Dependent Protein Kinases / metabolism*
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Endoplasmic Reticulum / metabolism
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In Situ Hybridization
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Inositol 1,4,5-Trisphosphate Receptors
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Membrane Proteins
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Mice
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Mice, Inbred C57BL
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Muscle, Smooth / metabolism
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Phosphoproteins / chemistry
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Phosphoproteins / metabolism*
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Protein Isoforms / genetics
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Protein Isoforms / metabolism*
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Receptors, Cytoplasmic and Nuclear / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Signal Transduction*
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Tissue Distribution
Substances
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Calcium Channels
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Inositol 1,4,5-Trisphosphate Receptors
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Membrane Proteins
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Mrvi1 protein, mouse
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Phosphoproteins
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Protein Isoforms
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Receptors, Cytoplasmic and Nuclear
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Recombinant Fusion Proteins
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Cyclic GMP-Dependent Protein Kinase Type I
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Cyclic GMP-Dependent Protein Kinases
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Prkg1 protein, mouse