Involvement of nitric oxide in spinally mediated hyperalgesia in the mouse

Neurosci Lett. 1992 Dec 14;148(1-2):1-5. doi: 10.1016/0304-3940(92)90790-e.

Abstract

Intrathecal injection of N-methyl-D-aspartate (NMDA) induces a short duration hyperalgesia in mice. An inhibitor of nitric oxide synthase (NOS), N omega-nitro-L-arginine methyl ester (L-NAME), administered either systemically or intrathecally, blocked the NMDA-induced hyperalgesia. This effect was partially reversed by the NOS substrate, L-arginine. Intrathecal hemoglobin mimicked the effects of L-NAME. Intrathecal injection of the NO-donating compounds, sodium nitroprusside (SNP) and hydroxylamine, resulted in a hyperalgesia that lasted 3 h and was reduced by coadministration of hemoglobin. Thus, nitric oxide production appears to mediate NMDA-induced hypersensitivity and may contribute to other forms of centrally induced hyperalgesia.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Oxidoreductases / antagonists & inhibitors
  • Animals
  • Arginine / administration & dosage
  • Arginine / analogs & derivatives*
  • Arginine / pharmacology
  • Cycloleucine / administration & dosage
  • Cycloleucine / analogs & derivatives
  • Cycloleucine / pharmacology
  • Hemoglobins / administration & dosage
  • Hemoglobins / pharmacology*
  • Hydroxylamine
  • Hydroxylamines / administration & dosage
  • Hydroxylamines / pharmacology
  • Hyperalgesia / physiopathology*
  • Ibotenic Acid / administration & dosage
  • Ibotenic Acid / analogs & derivatives
  • Ibotenic Acid / pharmacology
  • Injections, Spinal
  • Kainic Acid / administration & dosage
  • Kainic Acid / pharmacology
  • Male
  • Mice
  • Mice, Inbred ICR
  • N-Methylaspartate / pharmacology*
  • NG-Nitroarginine Methyl Ester
  • Neurotoxins / pharmacology
  • Nitric Oxide / metabolism*
  • Nitric Oxide Synthase
  • Nitroprusside / administration & dosage
  • Nitroprusside / pharmacology
  • Spinal Cord / drug effects
  • Spinal Cord / physiology*
  • Spinal Cord / physiopathology
  • Time Factors
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid

Substances

  • Hemoglobins
  • Hydroxylamines
  • Neurotoxins
  • Cycloleucine
  • 1-amino-1,3-dicarboxycyclopentane
  • Nitroprusside
  • Ibotenic Acid
  • Hydroxylamine
  • Nitric Oxide
  • N-Methylaspartate
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
  • Arginine
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases
  • Kainic Acid
  • NG-Nitroarginine Methyl Ester