Abstract
The contribution of peripheral macrophage was assessed in cuprizone intoxication, a model of demyelination and remyelination in which the blood-brain barrier remains intact. Flow cytometry of brain cells isolated from cuprizone-treated mice revealed an increase in the percentage of Mac-1(+)/CD45(hi) peripheral macrophage. To confirm these results in situ, C57BL/6 mice were lethally irradiated, transplanted with bone marrow from GFP-transgenic mice, and exposed to cuprizone. GFP(+) peripheral macrophages were seen in the CNS after 2 weeks of treatment, and infiltration continued through 6 weeks. While the peripheral macrophages were far outnumbered by the resident microglia, their recruitment across the blood-brain barrier alludes to a potentially important role.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Blood-Brain Barrier / drug effects
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Blood-Brain Barrier / immunology*
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Bone Marrow Cells / immunology
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Bone Marrow Transplantation
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CD3 Complex / immunology
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Central Nervous System / cytology
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Central Nervous System / immunology*
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Chemotaxis, Leukocyte / drug effects
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Chemotaxis, Leukocyte / immunology*
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Demyelinating Autoimmune Diseases, CNS / chemically induced
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Demyelinating Autoimmune Diseases, CNS / immunology*
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Demyelinating Autoimmune Diseases, CNS / physiopathology
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Disease Models, Animal
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Flow Cytometry
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Leukocyte Common Antigens / immunology
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Macrophage-1 Antigen / immunology
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Macrophages / cytology
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Macrophages / drug effects
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Macrophages / immunology*
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Radiation Chimera
Substances
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CD3 Complex
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Macrophage-1 Antigen
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Leukocyte Common Antigens