Abstract
The estrogen receptor plays a critical role in the pathogenesis and clinical behavior of breast cancer. To better understand the molecular basis of estrogen-dependent forms of this disease we studied gene expression profiles from 53 primary breast cancer biopsies. Gene expression data for more than 7000 genes were generated from each tumor sample with oligo microarrays. A standard correlation-clustering algorithm identified 18 genes that co-clustered with estrogen receptor alpha. Eleven of these genes had previously been associated with estrogen regulation or breast tumorigenesis including trefoil factor 1 and estrogen regulated LIV-1. Additional study of these 18 genes may further delineate the role of estrogen receptor in breast cancer, generate new predictive biomarkers for response to endocrine therapies and identify novel therapeutic targets.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 6 print issues and online access
$259.00 per year
only $43.17 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ponglikitmongkol M, Green S, Chambon P . Genomic organization of the human oestrogen receptor gene EMBO J 1988 7: 3385–3388
Williams MR, Todd JH, Ellis IO, Dowle CS, Haybittle JL, Elston CW et al . Oestrogen receptors in primary and advanced breast cancer: an eight year review of 704 cases Br J Cancer 1987 55: 67–73
Nomura Y, Miura S, Koyama H, Enomoto K, Kasumi F, Yamamoto H et al . Relative effect of steroid hormone receptors on the prognosis of patients with operable breast cancer. A univariate and multivariate analysis of 3089 Japanese patients with breast cancer from the Study Group for the Japanese Breast Cancer Society on Hormone Receptors and Prognosis in Breast Cancer Cancer 1992 69: 153–164
Andersen J, Thorpe SM, King WJ, Rose C, Christensen I, Rasmussen BB et al . The prognostic value of immunohistochemical estrogen receptor analysis in paraffin-embedded and frozen sections versus that of steroid-binding assays Eur J Cancer 1990 26: 442–449
Clark GM, McGuire WL . Steroid receptors and other prognostic factors in primary breast cancer Semin Oncol 1988 15: 20–25
Litherland S, Jackson IM . Antioestrogens in the management of hormone-dependent cancer Cancer Treat Rev 1988 15: 183–194
Elledge R, Green S, Pugh R, Allred DC, Clark GM, Hill J et al . Estrogen receptor (ER) and progesterone receptor (PgR), by ligand-binding assay compared with ER, PgR and pS2, by immunohistochemistry in predicting response to tamoxifen in metastatic breast cancer: a southwest oncology group study Int J Cancer (Pred Oncol) 2000 89: 111–117
Pritchard KJ . Current and future directions in medical therapy for breast carcinoma Cancer 2000 88: 3065–3072
Ribieras S, Tomasetto C, Rio MC . The pS2/TFF1 trefoil factor, from basic research to clinical applications Biochim Biophys Acta 1998 1378: F61–F77
Lockhart DJ, Dong H, Byrne MC, Follettie MT, Gallo MV, Chee MS et al . Expression monitoring by hybridization to high-density oligonucleotide arrays Nat Biotechnol 1996 14: 1675–1680
Coop A, Dressman M, Lavedan C, Singh B, Lombart-Cussac A, Miller WR et al . Correlation of immunohistochemistry (IHC) and gene microarray analysis of breast biopsies from a preoperative endocrine therapy trial (Abstract) Amer Soc Clin Onc 2001 3118
Molina R, Farrus B, Filella X, Jo J, Zanon G, Pahisa J et al . Carcinoembryonic antigen in tissue and serum from breast cancer patients relationship with steroid receptors and clinical applications in the prognosis and early diagnosis of relapse Anticancer Res 1999 19: 2557–2562
Zach O, Kasparu H, Krieger Q, Hehenwarter W, Girschikofsky M, Lutz D . Detection of circulating mammary carcinoma cells in the peripheral blood of breast cancer patients via a nested reverse transcriptase polymerase chain reaction assay for mammaglobin mRNA J Clin Oncol 1999 17: 2015–2019
El-Tanani MK, Green CD . Interaction between estradiol and growth factors in the regulation of specific gene expression in MCF-7 human breast cancer cells J Steroid Biochem Mol Biol 1997 60: 269–276
Clark JW, Snell L, Shiu RP, Orr FW, Maitre N, Vary CP et al . The potential role for prolactin-inducible protein (PIP) as a marker of humanbreast cancer micrometastasis Br J Cancer 1999 81: 1002–1008
GeneCard: PIP, Updated 9/4/2000 . htttp:bioinformatics. weizmann.ac.il/cards/
GeneCard: MGP, Updated 8/31/2000 . htttp:bioinformatics. weizmann.ac.il/cards/
Sheikh MS, Shao ZM, Chen JC, Fontana JA . Differential regulation of matrix Gla protein (MGP) gene expression by retinoic acid and estrogen in human breast carcinoma cells Mol Cell Endocrinol 1993 92: 153–160
LocusLink: 7031, Updated 12/31/2000 . http:www.ncbi.nlm.nih.gov/LocusLink/index.html
Efstathiou JA, Noda M, Rowan A, Dixon C, Chinery R, Jawhari A et al . Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells Proc Natl Acad Sci USA 1998 95: 3122–3127
May FE, Westley BR . Expression of human intestinal trefoil factor in malignant cells and its regulation by oestrogen in breast cancer cells J Pathol 1997 182: 404–413
Prud’homme JF, Fridlansky F, Le Cunff M, Atger M, Mercier-Bodart C, Pichon MF et al . Cloning of a gene expressed in human breast cancer and regulated by estrogen in MCF-7 cells DNA 1985 4: 11–21
Beck S, Sommer P, dos SS, Blin N, Gott P . Hepatocyte nuclear factor 3 (winged helix domain) activates trefoil factor gene TFF1 through a binding motif adjacent to the TATAA box DNA Cell Biol 1999 18: 157–164
Perou CM, Sorlie T, Eisen MB, van de Rijn M, Jeffrey SS, Rees CA et al . Molecular portraits of human breast tumours Nature 2000 406: 747–752
GeneCard: HPN, Updated 11/21/2000 . http:bioinformatics.weizmann.ac.il/cards/
Liou HC, Boothby MR, Finn PW, Davidon R, Nabavi N, Zeleznik-Le NJ et al . A new member of the leucine zipper class of proteins that binds to the HLA DR alpha promoter Science 1990 247: 1581–1584
Diez-Itza I, Sanchez LM, Allende MT, Vizoso F, Ruibal A, Lopez-Otin C . Zn-alpha 2-glycoprotein levels in breast cancer cytosols and correlation with clinical, histological and biochemical parameters Eur J Cancer 1993 29A: 1256–1260
GeneCard: AZGP1, Updated 8/31/2000 . http:bioinformatics.weizmann.ac.il/cards/
Bertucci F, Houlgatte R, Benziane A, Granjeaud S, Adelaide J, Tagett R et al . Gene expression profiling of primary breast carcinomas using arrays of candidate genes Hum Mol Genet 2000 9: 2981–2991
GeneCard: TGBR3, Updated 11/4/2000 . http:bioinformatics.weizmann.ac.il/cards/
OMIM: TGFB1, Updated 10/31/2000 . http:www.ncbi.nlm.nih.gov/Omim/searchomim.html
Bratt T . Lipocalins and cancer Biochim Biophys Acta 2000 1482: 318–326
Acknowledgements
We thank Dr Eric Holland for supplying the endothelial and glioblastoma cell pellets and Kristin Franczyk for manuscript preparation.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dressman, M., Walz, T., Lavedan, C. et al. Genes that co-cluster with estrogen receptor alpha in microarray analysis of breast biopsies. Pharmacogenomics J 1, 135–141 (2001). https://doi.org/10.1038/sj.tpj.6500022
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.tpj.6500022
Keywords
This article is cited by
-
An isomiR expression panel based novel breast cancer classification approach using improved mutual information
BMC Medical Genomics (2018)
-
ER and PR signaling nodes during mammary gland development
Breast Cancer Research (2012)
-
A network-based, integrative study to identify core biological pathways that drive breast cancer clinical subtypes
British Journal of Cancer (2012)
-
Changes in expression of oestrogen regulated and proliferation genes with neoadjuvant treatment highlight heterogeneity of clinical resistance to the aromatase inhibitor, letrozole
Breast Cancer Research (2010)
-
Mammary gland zinc metabolism: regulation and dysregulation
Genes & Nutrition (2009)