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Change history
29 May 2008
In the version of this correspondence initially published, the two previously published datasets analyzed were labeled with incorrect references in Figure 1b. Reference 2 should be associated with the second column (80 sites) and Reference 3 should be associated with the third column (96 sites). The weblink for the Zinc Finger Consortium website was incorrect and should be http://www.zincfingers.org/. The errors have been corrected in the HTML and PDF versions of the article.
References
Porteus, M.H. & Carroll, D. Nat. Biotechnol. 23, 967–973 (2005).
Segal, D.J. et al. Biochemistry 42, 2137–2148 (2003).
Bae, K.H. et al. Nat. Biotechnol. 21, 275–280 (2003).
Mandell, J.G. & Barbas, C.F. Nucleic Acids Res. 34, W516–W523 (2006).
Liu, Q., Xia, Z., Zhong, X. & Case, C.C. J. Biol. Chem. 277, 3850–3856 (2002).
Wright, D.A. et al. Nat. Protoc. 1, 1637–1652 (2006).
Acknowledgements
We thank D. Dobbs and P. Zaback for helpful discussions and E. Unger-Wallace (Iowa State University) for contributions to zinc-finger array construction. J.K.J. is supported by the US National Institutes of Health (R21 RR024189), the Cystic Fibrosis Foundation (MCCRAY07G0) and the Massachusetts General Hospital Department of Pathology; D.F.V. is supported by a US National Science Foundation grant DBI 0501678; T.C. is supported by the German Research Foundation (SPP1230 CA311/2); C.L.R. is supported by a Ford Foundation Pre-Doctoral Diversity Fellowship and a National Science Foundation Graduate Research Fellowship; T.I.C. is supported by a fellowship from the Swiss Foundation for Grants in Biology and Medicine.
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Ramirez, C., Foley, J., Wright, D. et al. Unexpected failure rates for modular assembly of engineered zinc fingers. Nat Methods 5, 374–375 (2008). https://doi.org/10.1038/nmeth0508-374
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DOI: https://doi.org/10.1038/nmeth0508-374
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