Archival ReportGlycine Transporter-1 Blockade Leads to Persistently Reduced Relapse-like Alcohol Drinking in Rats
Section snippets
Animals
Fifty-eight 2-month-old male Wistar rats (from our own breeding colony at the Central Institute of Mental Health, Mannheim, Germany) were used for the ADE and gene expression experiments. For further information, see Supplementary Methods and Materials in Supplement 1.
Long-Term Alcohol Self-Administration with Repeated Deprivation Phases
The long-term voluntary alcohol-drinking procedure excluding all deprivation phases lasted a total of 52 weeks (see Supplementary Methods and Materials in Supplement 1 for detailed information).
The pharmacological studies were
Effect of the Administration of Org25935 on ADE Measurements
Following the reintroduction of alcohol solutions after the eighth deprivation phase, the vehicle-treated group showed a typical increase in alcohol consumption, indicating the occurrence of an ADE (Figure 1A,B). This increase was not different from that observed during the first seven deprivation phases (data not shown). With respect to the pharmacological treatment, a two-way ANOVA for repeated measures revealed significantly different alcohol intake after a deprivation phase in all animal
Discussion
In the present study, we demonstrated that administration of the glycine transporter GlyT1 blocker Org25935 caused a significant dose-dependent reduction of relapse-like alcohol consumption in male Wistar rats. This effect was comparable with that seen in acamprosate-treated rats. Following both acamprosate and Org25935 treatment, water intake during ADE measurement was significantly increased, indicating the selectivity of these treatments toward alcohol consumption. However, the effect of the
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Authors VV and FL-E contributed equally to this work.