Elsevier

Biochemical Pharmacology

Volume 75, Issue 4, 15 February 2008, Pages 942-955
Biochemical Pharmacology

Regulation of cyclic AMP response-element binding-protein (CREB) by Gq/11-protein-coupled receptors in human SH-SY5Y neuroblastoma cells

https://doi.org/10.1016/j.bcp.2007.10.015Get rights and content
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Abstract

Human SH-SY5Y neuroblastoma cells have been used to investigate mechanisms involved in CREB phosphorylation after activation of two endogenously expressed Gq/11-protein-coupled receptors, the M3 muscarinic acetylcholine (mACh) and B2 bradykinin receptors. Stimulation with either methacholine or bradykinin resulted in maximal increases in CREB phosphorylation within 1 min, with either a rapid subsequent decrease (bradykinin) to basal levels, or a sustained response (methacholine). Inhibitor studies were performed to assess the involvement of a number of potential kinases in signalling to CREB phosphorylation. Removal of extracellular Ca2+, inhibition of Ca2+/calmodulin-dependent protein kinase II and down-regulation of protein kinase C (PKC) resulted in reduced CREB phosphorylation after both M3 mACh and B2 bradykinin receptor activation. In contrast, inhibition of MEK1/2 by U0126 resulted in significantly reduced CREB phosphorylation levels after B2 bradykinin, but not M3 mACh receptor activation. In addition, we demonstrate that maintained phosphorylation of CREB is necessary for CRE-dependent gene transcription as the M3 mACh, but not the B2 bradykinin receptor activates both a recombinant CRE-dependent reporter gene, and the endogenous c-Fos gene. These data highlight the involvement of multiple, overlapping signalling pathways linking these endogenous Gq/11-coupled metabotropic receptors to CREB and emphasize the importance of the duration of signalling pathway activation in converting a CREB phosphorylation event into a significant change in transcriptional activity.

Abbreviations

CREB
cyclic AMP response-element binding-protein
ERK
extracellular signal-regulated kinase
GPCR
G-protein-coupled receptor
IBMX
3-isobutyl-1-methylxanthine
mACh
muscarinic acetylcholine
MAPK
mitogen-activated protein kinase
PDBu
phorbol 12,13-dibutyrate
PKA
cyclic AMP-dependent protein kinase
PKC
protein kinase C
PLC
phospholipase C

Keywords

Cyclic AMP response-element binding-protein (CREB)
Muscarinic acetylcholine receptor
Bradykinin receptor
SH-SY5Y cells
Intracellular calcium concentration [Ca2+]i
Calmodulin
Ca2+/calmodulin-dependent protein kinase
Protein kinase C
c-Fos

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