Biochemical and Biophysical Research Communications
Antiangiogenic activity of 4-O-methylgallic acid from Canavalia gladiata, a dietary legume
Section snippets
Materials and methods
General procedures. The HREI-MS spectra were obtained using a Jeol JMS-700 mass spectrometer. The 1H and 13C NMR spectra were determined on Brucker AC 500 (500 MHz for 1H and 125 MHz for 13C) in CDCl3. All chemical shifts were recorded according to an internal Me4Si. Analytical thin-layer chromatography (TLC) was performed on Merck Silicagel 60 F254 aluminum plates. All solvents used were of spectral grade or distilled prior to use.
Isolation of 4-OMGA. The dried seeds of C. gladiata were milled
Isolation and structure determination of 4-OMGA
Bioactivity-guided isolation of 4-OMGA was performed through the inhibitory activity of seed extract against the proliferation of BAECs [11], [12], [13]. BAECs treated with each solvent fraction from the seed extract were incubated for 3 days and MTT colorimetric assay was conducted. As a result, ethylacetate-soluble fraction showed notable activity on the assay. Thereafter, the active compound was purified from the ethylacetate-soluble fraction as described under Materials and methods.
The
Acknowledgments
This study was supported by grants from the National Research Laboratory (2004-02405) and the Brain Korea 21 project (C049).
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