SMFM meeting paperGender-specific effect of overexpression of sFlt-1 in pregnant mice on fetal programming of blood pressure in the offspring later in life
Section snippets
Animals
Pregnant CD-1 mice at day 6 of gestation were purchased from Charles River (Wilmington, MA). The mice were housed separately in temperature- and humidity-controlled quarters with constant 12-hour light:dark cycles and provided with food and water ad libitum. At day 8 of gestation, pregnant CD-1 mice were divided randomly into 2 groups and injected through the tail vein with either adenovirus carrying sFlt-1 (109 plaque-forming units in 100 μL; sFlt-1 group) or adenovirus carrying the murine
Weight after weaning
The male and female offspring from sFlt-1– and mFc-treated pregnant mice were followed until adulthood. The weight from weaning until adulthood of male offspring that were born to sFlt-1–treated pregnant mice was significantly lower than male offspring that were born to mFc-treated pregnant mice (Figure 1A). There was no significant difference in the postweaning weight in female offspring between the 2 groups (Figure 1B).
BP results in male offspring
Mean BP was significantly higher during the entire measurement period in
Comment
We found that systolic, diastolic, and mean BPs of male offspring at 6 months of age that were born to pregnant mothers that were injected with adenovirus carrying sFlt-1 were significantly higher than the similarly aged male offspring from mFc-treated pregnant mothers (adenovirus carrying the murine immunoglobulin G2α Fc fragment as a control) and that these differences were not present in female offspring at the same age that were born to sFlt-1– and mFc-treated mothers. There were no
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Cite this article as: Lu F, Bytautiene E, Tamayo E, et al. Gender-specific effect of overexpression of sFlt-1 in pregnant mice on fetal programming of blood pressure in the offspring later in life. Am J Obstet Gynecol 2007;197:418.e1-418.e5.