Simulation of human xenobiotic metabolism in microorganisms: Yeast a good compromise between E. coli and human cells

doi:10.1016/S0168-8278(97)80500-4

Journal of Hepatology

Volume 26, Supplement 2, 1997, Pages 81-85

https://doi.org/10.1016/S0168-8278(97)80500-4 Get rights and content

Abstract

An overview of current heterologous expression systems for xenobiotic metabolising enzymes is given with a special emphasis on the yeast expression system. In a first part, basic properties and relative advantages and drawbacks of each expression system are considered. The second part is dedicated to humanized yeast strains allowing human P450 expression in a tailored redox environment and to the possibility to use such strains to simulate complex metabolisms involving a combination of phase I and phase II reactions. The last part presents how the association of numeric simulation to yeast expression can help in understanding rules controlling metabolic profiles in xenobiotic-acting multienzymatic systems.

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Journal of Hepatology

Abstract

References (19)

Recombinant DNA approaches for the development of metabolic systems used in in vitro toxicology

Mutation Res

Novel exogenous heme-dependent expression of mammalian cytochrome P450 using baculovirus

Arch Biochem Biophys

Expression of mammalian cytochrome P450 using vaccinia virus

Methods Enzymol

Expression of cytochrome P450 cDNAs in human B lymphoblastoid cells, applications to toxicology and metabolite analysis

Methods Enzymol

Enhanced in vivo monooxygenase activities of mammalian P450s in engineered yeast cells producing high levels of NADPH-cytochrome P450 reductase and human cytochrome b5

Gene

The P450 superfamily: update on new sequences, gene mapping, accession numbers, early trivial names of enzymes, and nomenclature

DNA Cell Biol

Heterologous expression of mammalian P450 enzymes

Adv Enzymol Mol Biol

Human cells as an expression system for cytochromes P450

Expression of mammalian cytochrome P450 enzymes using yeast-based vectors

Methods Enzymol

Cited by (0)