A dose-response relationship between exposure to cockroach allergens and induction of sensitization in an experimental asthma in Hartley guinea pigs☆,☆☆,★,★★
Section snippets
Animals
Male Hartley strain guinea pigs weighing 200 to 250 gm were purchased from Hilltop Laboratories (Scotsdale, Pa.). Upon arrival, two animals were housed in a plastic cage in a colony room with a 12-hour day-night cycle (lights on at 7 am) through artificial illumination. They received food and water ad libitum. Before the start of the experiment, guinea pigs were quarantined and acclimatized to their housing for 1 week. The experiment was carried out with the approval of the Institutional Animal
Anaphylactic sensitization
As shown in Table I, the saline-sensitized guinea pigs (control) showed negative reactions to the CRa skin test, whereas in the CRa-sensitized guinea pigs, LD, MD, or HD revealed positive reactions (p < 0.001).
Group Saline CRa 0.5 μg CRa 1 μg CRa 5 μg Histamine Control (n = 11) 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.3 ± 0.2 12.3 ± 0.4 LD (n = 6) 0.0 ± 0.0 7.6 ± 0.3 9.2 ± 0.7 12.5 ± 0.3 14.3 ± 0.3 MD (n = 6) 0.0 ± 0.0 7.5 ± 0.6 10.0 ± 0.6 12.4 ± 0.6 12.8 ± 0.3 HD (n = 6) 0.0 ± 0.0 7.4 ± 0.2 11.0 ± 0.2
Discussion
This study further demonstrates that aerosolized CRa exposure alone sensitizes animals to CRa and that the sensitized animals develop an antigen-specific “asthma-like” airway obstruction on CRa inhalation. This confirms previous studies from this laboratory.14, 15 Moreover, the anaphylactic sensitization appears to be correlated to the levels of CRa exposures of the guinea pigs during the sensitization period (Fig. 1 and Table II, Table III). However, the airway obstruction as manifested by the
Acknowledgements
We thank Mrs. Betsy Dennis for word processing assistance, James E. Burgett for editorial assistance, and Dr. Mary K. Rayens for assistance in data statistical analysis.
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Cited by (0)
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From athe Division of Allergy and Immunology, the Department of Internal Medicine, and bthe Department of Physiology, University of Kentucky, Lexington.
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Supported by the Center for Indoor Air Research.
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Reprint requests: Daohong Zhou, MD, Division of Allergy and Immunology, Department of Internal Medicine, University of Kentucky, 800 Rose St., Lexington, KY 40536.
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