Elsevier

Life Sciences

Volume 61, Issue 26, 21 November 1997, Pages 2567-2574
Life Sciences

A COMPARISON OF HEPATIC CYTOCHROME P450 PROTEIN EXPRESSION BETWEEN INFANCY AND POSTINFANCY

https://doi.org/10.1016/S0024-3205(97)01011-4Get rights and content

Abstract

We immunochemically measured the contents of 9 different cytochrome P450 (CYP) isoenzymes expressed in the liver and compared them between two groups: one group of 6 infant and 4 perinatal patients and one group of 10 patients after infancy (over 1 year old). CYP protein expressed in human liver can be divided into three groups on the basis of expression pattern: (a) CYP2A6, 2C9, 2D6, 2E1, and 3A were present in all samples and no difference was observed between the two groups; (b) CYP1A2, 2B6, and 2C8 were expressed more after infancy than during infancy; and (c) CYP3A7, which has been considered a major CYP enzyme in fetal liver microsomes, was expressed in all infants as well as the four perinatal patients, whereas it was detected in only 2 patients after infancy. These results implied that CYP2A6, 2C9, 2D6, 2E1, and 3A are already expressed during perinatal and infant period, while CYP1A2, 2B6, and 2C8 are expressed highly in subjects over 1 year old, and CYP3A7 disappeared after infancy.

Section snippets

Patients and Methods

The human liver samples (except Nos 18 and 19) were collected postmortem from Japanese patients at the National Institute of Neuroscience after obtaining written informed consent from the patient's family. Two liver samples (Nos 18 and 19) were obtained from Japanese patients undergoing liver resection after obtaining written informed consent from the patient. Table 1 summarizes the sex, age, and causes of abortion, death, or surgery. Samples were obtained from portions without particular

Results

Representative Western blot results of sample Nos 4, 9, 17, and 19, and the reference standards are shown in Fig. 1. The contents of total CYPs determined spectrally and each CYP expressed in the liver of Perinatal/Infant Group and Control Group are summarized in Table 2. A good correlation was found between the contents of total CYPs measured spectrally and the sum of each CYP determined immunochemically (r=0.6764, p<0.005). The recoveries of sum of each CYP determined immunochemically

Discussion

We immunochemically measured the contents of each CYP protein expressed in the liver and compared them between the perinatal/infant patients (under 1 year old) and patients over 1 year old. Since previous studies on CYP1A2 showed lower expression and activity in the neonatal period 5, 6, 7, 8, we focused on CYP expression under 1 year of age and compared it to the control group in which samples were collected from patients at various ages so as to avoid a specific age bias. Although a good

Acknowledgements

We thank Dr. Susumu Yamaguchi and Dr. Hitonobu Moriya for their help with obtaining liver samples.

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