Sepiapterin reductase from horse liver: Purification and properties of the Enzyme1
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Cited by (59)
Uterine secretome: What do the proteins say about maternal-fetal communication in buffaloes?
2024, Journal of ProteomicsSepiapterin reductase mediates chemical redox cycling in lung epithelial cells
2013, Journal of Biological ChemistryCitation Excerpt :We found that these point mutations caused major losses (90–98%) in the ability of sepiapterin reductase to reduce sepiapterin confirming that in the wild type enzyme these amino acids bind tightly to NADPH, generating a unique structural conformation that is essential for substrate reduction. These data are also consistent with the requirement for the adenine nucleotide for optimal enzyme activity (25). Interestingly, NADPH-binding site mutant sepiapterin reductase enzymes still retained 25–55% of their redox cycling activity.
Membrane transport of sepiapterin and dihydrobiopterin by equilibrative nucleoside transporters: A plausible gateway for the salvage pathway of Tetrahydrobiopterin biosynthesis
2011, Molecular Genetics and MetabolismCitation Excerpt :The coenzyme is converted to inactive 7,8-dihydrobiopterin (7,8BH2 or simply BH2 ) through spontaneous isomerization of qBH2. It has long been known that BH4 is produced from sepiapterin (SP; 6-lactyl-7,8-dihydropterin) by two distinct enzymes, sepiapterin reductase (EC 1.1.1.153, Km to SP, 21 μM [10]) and dihydrofolate reductase (DHFR, EC 1.5.1.3, Km to 7,8BH2, 5–15 μM [11,12]), the latter of which converts 7,8BH2 to active BH4 [11,13–15]. The route of BH4 retrieval from SP or BH2 was termed the “salvage pathway” as opposed to the de novo pathway of BH4 biosynthesis [16].
The silkworm mutant lemon (lemon lethal) is a potential insect model for human sepiapterin reductase deficiency
2009, Journal of Biological ChemistryCitation Excerpt :The blot was visualized using Western Lightning Chemiluminescence Reagent Plus (PerkinElmer Life Sciences) and a LAS 1000 imaging system (Fuji Film). Enzyme Assay—SPR activity was assayed according to the method reported by Katoh (21) with slight modifications. Ten micrograms of protein from whole body or 0.1-0.5 μg of recombinant BmSPR was used in the assessment.
Cellular uptake of sepiapterin and push-pull accumulation of tetrahydrobiopterin
2008, Molecular Genetics and MetabolismA brain-specific decrease of the tyrosine hydroxylase protein in sepiapterin reductase-null mice-as a mouse model for Parkinson's disease
2008, Biochemical and Biophysical Research Communications
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A part of this work was presented at the 4th International Symposium on Pteridines, Toba, July 21–25, 1969.